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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

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This model clarifies key mechanisms underlying aspirin-associated enteropathy (162)

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And I sort of had a hypothesis that, you know, obesity, causes DNA damage through double-stranded breaks and that potentially, you know, because people that are obese are eating terrible diets, obviously theres all sorts of refined sugar and terrible things that theyre adding

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